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Widely-Targeted Metabolomics for Plants: A Comprehensive Overview

Plants produce a vast array of metabolites that play a crucial role in regulating growth, development, and responses to environmental stress. Analyzing these metabolites can provide valuable insights into plant metabolism and potential applications in various fields. In this blog, we will explore the significance of widely-targeted metabolomics for plants and the different techniques used in this field.

What is Metabolomics?

Metabolomics is the systematic study of the complete set of metabolites present in biological systems. Metabolites are small organic molecules that are intermediates or end products of cellular metabolism. Metabolomics techniques allow the analysis of metabolite profiles and provide insights into metabolic pathways, biochemical interactions, and the function of cellular networks.

Widely-Targeted Metabolomics for Plants

Widely-targeted metabolomics is a comprehensive approach to identify and quantify a wide range of metabolites in a biological sample. Unlike targeted metabolomics, which focuses on specific metabolites, widely-targeted metabolomics utilizes non-selective separation techniques, such as liquid chromatography and gas chromatography-mass spectrometry, to analyze a broad spectrum of metabolites.

Widely-targeted metabolomics offers a holistic approach to characterize plant metabolism. Enabling the identification of novel metabolic pathways, biomarkers, and potential applications in agriculture, pharmaceuticals, and biotechnology.

Widely-Targeted Metabolomics Techniques for Plants

Liquid Chromatography-Mass Spectrometry (LC-MS): LC-MS is a widely used technique for metabolomics analysis in plants. It involves the separation of metabolites by liquid chromatography followed by ionization and mass analysis in a mass spectrometer. LC-MS can detect a wide range of metabolites, including amino acids, nucleotides, lipids, and secondary metabolites.

Gas Chromatography-Mass Spectrometry (GC-MS): GC-MS is another powerful technique for metabolomics analysis, particularly for volatile and semi-volatile metabolites. It involves separation based on volatility followed by mass analysis in a mass spectrometer. GC-MS can detect a broad spectrum of metabolites, including organic acids, sugars, and fatty acids.

Nuclear Magnetic Resonance (NMR) Spectroscopy: NMR spectroscopy is a non-destructive technique that provides detailed information on the structure and quantity of metabolites. It involves measurement of the magnetic properties of metabolites, which reflect their chemical environment. NMR can detect a wide range of metabolites, including amino acids, sugars, and organic acids.

Direct Infusion Mass Spectrometry (DIMS): DIMS is a rapid, non-selective technique that involves the direct infusion of sample extracts into a mass spectrometer. This approach can detect a wide range of metabolites, including complex glycosides and lipids.

Applications of Widely-Targeted Metabolomics for Plants

Plant Metabolism: Widely-targeted metabolomics provides insights into the complexity of plant metabolism, facilitating the identification of regulatory pathways, metabolic switches. And novel metabolites.

Plant-Microorganism Interactions: Metabolomics analysis can elucidate the role of metabolites in plant-microorganism interactions. Providing insights into plant defense mechanisms and symbiosis.

Crop Improvement: Metabolomics analysis can identify biomarkers related to plant growth, yield. And stress tolerance, facilitating the development of improved crops.

Drug Discovery: Plant metabolites have potential applications in pharmaceuticals and drug discovery. Widely-targeted metabolomics can identify novel bioactive compounds with pharmacological properties.

Challenges and Future Directions

Widely-targeted metabolomics for plants faces several challenges, including the complexity of metabolite extraction and analysis, chemical diversity. And the lack of standardized protocols. The future direction of this field involves the integration of multi-omics approaches. Such as genomics, proteomics, and transcriptomics, for a more comprehensive understanding of plant metabolism.

In conclusion, widely-targeted metabolomics is a powerful tool for plant biology research. Providing crucial insights into the complex metabolic pathways and networks that govern plant growth and development. The application of this technique has potential benefits in agriculture, biotechnology. And pharmaceuticals, making it an exciting area of research with promising prospects.

About the author

Collected by Lifeasible, a biotechnology company specialized in agricultural field. Lifeasible use LC-MS/MS technology to perform broadly targeted quantitative metabolomic detection and analysis of plant metabolites. The widely targeted metabolomics services provided by Lifeasible for plant metabolites combines non-targeted technology with targeted MRM technology. Featuring as able to identifying multiple substances, accurate characterization and quantification, wide dynamic range, high sensitivity, and good reproducibility.

Lifeasible, a leading provider of metabolomics solutions, today announced the update of its Phytohormone Targeted Metabolomics Assay. This enhanced service enables researchers to quantify an expanded panel of plant hormone compounds with unparalleled precision. Further advancing plant biology research and crop improvement.

Plant hormones play a central role in regulating plant growth, development, and responses to environmental stimuli. Lifeasible’s updated targeted metabolomics assay provides researchers with a powerful tool to elucidate the complex interplay of plant hormones and their role in various biological processes.

“We are excited to offer this updated assay to the plant research community,” said Isla. One of the representative speakers of Lifeasible. “Our team has worked diligently to expand the coverage of our assay. Providing researchers with even more insights into plant metabolism and development.”

Lifeasible’s updated assay offers

The updated Phytohormone Targeted Metabolomics Assay leverages Lifeasible’s advanced mass spectrometry platform. And proprietary databases to achieve precise identification and quantification of an expanded panel of plant hormones. The assay covers a broad range of hormone classes. Including newly discovered plant hormones involved in defense responses and other physiological processes.

Lifeasible’s updated assay offers several advantages over existing methods. The use of isotope internal standards ensures accurate and reliable quantification. A rigorous manual proofreading process guarantees the accuracy of results. Additionally, Lifeasible provides comprehensive bioinformatic analysis, including PCA, OPLS-DA, and analysis of variance, to help researchers interpret complex metabolomics data.

The applications of this updated technology are vast. Researchers can use the assay to investigate plant hormone signaling pathways, elucidate the mechanisms of plant stress responses, and identify key regulators of crop yield and quality. Plant breeders can leverage the technology to develop crops with improved traits. Such as enhanced disease resistance or increased nutritional content.

Lifeasible is committed to advancing plant biology research and promoting global food security,” said Isla. “We believe our updated targeted metabolomics assay will further empower researchers to make breakthrough discoveries that can transform agriculture.”

Source from Lifeasible

As a biotechnology company, Lifeasible is specialized in agricultural science. Offering a wide variety of agro-related services and products for environmental and energy solutions.

Our plant breeding and culture services support increasingly stringent safety and quality standards in the agricultural industry. Relying on our revolutionary techniques, various molecular breeding services and molecular diagnostic methods are offered for a wider range of agriculture-related sectors. Meanwhile, extensive analytical solutions reach out to a wider community of researchers in environmental and energy fields. Holding a strong tie between biological innovation and transformation in the ecosystem, Lifeasible now leverages the expertise. And strengths of each to create its unique platform that is accessible to all leaders working in agriculture, botany, biology, ecology. Having taken on step forward, we also provide lab-to-field solutions that advance the breeding process for farmers and breeders. Diverse analytical tools are available for breeding or quality control purposes in identification and production of plants.

Lifeasible welcomes collaborative relationships with other parties of interest, enabling flexible and rapid development of new technologies and products. Our staff is always ready to discuss the details of specific needs and to develop strategies tailored to your requirements.

Ed Jones

As a biotechnology company, Lifeasible is specialized in agricultural science, offering a wide variety of agro-related services and products for environmental and energy solutions.

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